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Askwith, W

10.1111/j.1600-0501.2010.01975.x 132 YamadaM.TsukimuraN.IkedaT.SugitaY.AttW.KojimaN.et al (2013)

Cells were washed twice with 10 ml 5% mannitol (Sigma-Aldrich), the mannitol was carefully and completely removed before 0.9 ml 100% methanol was added onto the cells, firmly rocking the dish so that the methanol covers the cell monolayer